Abstract
Purpose – To determine if metabolic and physiological changes were produced by participants wearing the LifeWave non-transdermal X39 phototherapy patch.
Cortisol levels were obtained from 8am, 12pm, 4pm, 8pm and 12am time collection saliva
Samples were purified with Phenomenex Strata polymeric reversed phase columns and analyzed
on Sciex 4000 Triple Quadrupole LC-MS/MS Mass Spectrometer using Phenomenex Kinetex C8
3x100mm, 2.6um, 100A column with linear gradient.
Dehydroepiandrosterone sulfate (DHEAS) levels were obtained at 8am, 8pm and 12am time
collections using Pantex salivary cortisol ELISA kit on Dynex DSX 4-plate automated ELISA
processing system.
Materials
– LifeWave X39 non-transdermal patches, Sabre Sciences urine and saliva test kits.
– LifeWave X39 non-transdermal patches, Sabre Sciences urine and saliva test kits.
Amino Acid panel was performed using a TSQ Quantum Triple Quadrupole LC-MS/MS Mass
Spectrometer. Adrenal stress panel, cortisol and DHEAS, from saliva were performed using a Sciex
4000 Triple Quadrupole LC-MS/MS Mass Spectrometer and Dynex DSX 4-plate automated ELISA
processing system respectively. Physiology testing was done using a Thought Technology
Biograph Infinity Physiology Suite with Cardio Pro software. Blood pressure was taken with a Model
#BPAG1-20CVS monitor. Questionnaires included: Marlow-Crowne, Arizona Integrative Outcome
Scale for Vitality, Pittsburg Sleep Quality Index, McGill Pain Scale, WAIS III- memory subtest,
Spectrometer. Adrenal stress panel, cortisol and DHEAS, from saliva were performed using a Sciex
4000 Triple Quadrupole LC-MS/MS Mass Spectrometer and Dynex DSX 4-plate automated ELISA
processing system respectively. Physiology testing was done using a Thought Technology
Biograph Infinity Physiology Suite with Cardio Pro software. Blood pressure was taken with a Model
#BPAG1-20CVS monitor. Questionnaires included: Marlow-Crowne, Arizona Integrative Outcome
Scale for Vitality, Pittsburg Sleep Quality Index, McGill Pain Scale, WAIS III- memory subtest,
Profile of Mood States.
Method
– Fifteen subjects of convenience age 40-65 were recruited through flyers and email.
Subjects were consented and baseline measures were taken. Patches were worn 8-12 hours each
day placed at either the GV14 or CV6 acupuncture points as selected by a computerized algorithm
and selected for ease and clarity of patch placement for participants. All measures were taken at all
test points: Baseline/day1, day 2 and day 7. Participants were asked to apply patch one hour prior
to testing on day 2 and 7.
– Fifteen subjects of convenience age 40-65 were recruited through flyers and email.
Subjects were consented and baseline measures were taken. Patches were worn 8-12 hours each
day placed at either the GV14 or CV6 acupuncture points as selected by a computerized algorithm
and selected for ease and clarity of patch placement for participants. All measures were taken at all
test points: Baseline/day1, day 2 and day 7. Participants were asked to apply patch one hour prior
to testing on day 2 and 7.
Cortisol levels were obtained from 8am, 12pm, 4pm, 8pm and 12am time collection saliva
Samples were purified with Phenomenex Strata polymeric reversed phase columns and analyzed
on Sciex 4000 Triple Quadrupole LC-MS/MS Mass Spectrometer using Phenomenex Kinetex C8
3x100mm, 2.6um, 100A column with linear gradient.
Dehydroepiandrosterone sulfate (DHEAS) levels were obtained at 8am, 8pm and 12am time
collections using Pantex salivary cortisol ELISA kit on Dynex DSX 4-plate automated ELISA
processing system.
Urine samples were taken by 10am at each test point. WAIS III data was taken immediately, at
10 minutes and at 20 minutes. A full 10 number sequence was used which was randomly generated
for each subject at each data point. Amino acid analysis was obtained on TSQ Quantum Triple
Quadrupole LC-MS/MS Mass Spectrometer using Phenomenex EzFaast amino acid kit.
10 minutes and at 20 minutes. A full 10 number sequence was used which was randomly generated
for each subject at each data point. Amino acid analysis was obtained on TSQ Quantum Triple
Quadrupole LC-MS/MS Mass Spectrometer using Phenomenex EzFaast amino acid kit.
Results
– Significant changes were seen in most essential and nonessential amino acids over the
– Significant changes were seen in most essential and nonessential amino acids over the
period of one week. These changes include:
- Changes of inflammatory markers and their metabolites
- Improvement in sleep quality
- Reduction in blood pressure
- Improvement in short term memory
- Improvement in reported feelings of vitality
Conclusion
– While this is a small sample of convenience the positive results in this study suggest
that further investigation needs to be done. Both the effect that appears to produce a number of
significant amino acid changes in a short period of time and the improvement in short term memory
which are particularly relevant to an ageing population and should be explored.
– While this is a small sample of convenience the positive results in this study suggest
that further investigation needs to be done. Both the effect that appears to produce a number of
significant amino acid changes in a short period of time and the improvement in short term memory
which are particularly relevant to an ageing population and should be explored.